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axio observer z 1 inverted epifluorescence microscope  (Carl Zeiss)


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    Carl Zeiss axio observer z 1 inverted epifluorescence microscope
    Axio Observer Z 1 Inverted Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4858 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+z+1+inverted+epifluorescence+microscope/Inverted+microscope+Axio+Observer+3/bio_rxiv__64898__2025__12__19__695352-230-25-24
    Average 99 stars, based on 4858 article reviews
    axio observer z 1 inverted epifluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Inverted Epifluorescence:

    Article Title: Function and firing of the Streptomyces coelicolor contractile injection system requires the membrane protein CisA
    Article Snippet: .. Images were acquired using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7 LED light source, and a Hamamatsu Orca Flash 4.0v3 sCMOS camera. mCherry fluorescence was detected using an excitation/emission bandwidth of 577–603 nm/614–659 nm. .. Images were collected using Zen Blue (Zeiss) and analyzed using Fiji ( ).

    Article Title: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
    Article Snippet: The band intensities for Cis1a and Cis2 were normalized against the band intensity of WhiA and plotted in GraphPad Prism (v9.3.1) with the mean ± s.d. .. For imaging protein localization and fluorescent promoter reporter fusion in S. coelicolor , a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with an sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera and a temperature-controlled incubation chamber was used. .. Images were acquired using a Zeiss Alpha Plan-Apo ×100/1.46 Oil DIC M27 objective with YFP excitation/emission bandwidths of 489–512 nm/520–550 nm.

    Article Title: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
    Article Snippet: The band intensities for Cis1a and Cis2 were normalized against the band intensity of WhiA and plotted in GraphPad Prism (Version 9.3.1) with the standard deviation. .. For imaging protein localization and fluorescent promoter reporter fusion in S. coelicolor , a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera, and a temperature-controlled incubation chamber was used. .. Images were acquired using a Zeiss Alpha Plan-Apo 100x/1.46 Oil DIC M27 objective with a YFP excitation/emission bandwidths of 489–512 nm/520–550 nm.

    Article Title: Bidirectional pilus processing in the Tad pilus system motor CpaF
    Article Snippet: .. Cells were immobilised on 1 % PYE agarose pads and imaged using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope equipped with a sCMOS camera (Hamamatsu Orca FLASH 4) and Zeiss Colibri 7 LED light source. .. Images were captured with a Zeiss Plan Apochromat 100x/NA 1.4 Ph3 objective lens and a GFP filter set (excitation: 488 nm, emission: 500–549 nm) using an exposure time of 250 ms.

    Article Title: Bidirectional pilus processing in the Tad pilus system motor CpaF.
    Article Snippet: .. Cells were immobilised on 1% PYE agarose pads and imaged using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope equipped with a sCMOS camera (Hamamatsu Orca FLASH 4) and Zeiss Colibri 7 LED light source. .. Images were captured with a Zeiss Plan Apochromat 100x/NA 1.4 Ph3 objective lens and a GFP filter set (excitation: 488 nm, emission: 500–549 nm) using an exposure timeof 250ms.

    Article Title: DNA damage-induced block of sporulation in Streptomyces venezuelae involves downregulation of ssgB .
    Article Snippet: Received 14 March 2022; Accepted 09 May 2022; Published 15 June 2022 Author affiliations: Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario, M5G 1M1, Canada; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada; Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.. *Correspondence: Susan Schlimpert, Susan.. Schlimpert@ jic. ac. uk; Justin R. Nodwell, justin. nodwell@ utoronto. ca

    Article Title: A bacterial CARD-NLR immune system controls the release of gene transfer agents
    Article Snippet: Protein loading controls were run on 12% SDS-PAGE gels and stained with an InstantBlue Coomassie protein stain (Abcam). .. Bacteria were immobilised on 1% agarose pads and visualised under a Zeiss Axio Observer Z.1 inverted epifluorescence microscope equipped with a Zeiss Plan Apochromat 100x/NA 1.4 Ph3 objective lens, an sCMOS camera (Hamamatsu Orca FLASH 4), and a Zeiss Colibri 7 LED light source. ..

    Article Title: A Novel Stress Response Pathway Mediates Antibiotic Tolerance and Architecture in Pseudomonas aeruginosa Biofilms
    Article Snippet: .. Time-lapse imaging was started from the beginning of the experiment and images were acquired every 5 min. P. aeruginosa strains were visualized using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7 LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera, and a temperature-controlled incubation chamber. .. Bacterial cells were loaded into B04A microfluidic plates (ONIX, CellASIC), grown by perfusing LB for 6 h in the case of PAO1 WT and Δ batR strains; or perfusing LB for 1 h, then switched to 0.1 mM IPTG LB for 9 h, and LB for 1h in the case of PAO1 strains overexpressing srkA .

    Fluorescence:

    Article Title: Function and firing of the Streptomyces coelicolor contractile injection system requires the membrane protein CisA
    Article Snippet: .. Images were acquired using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7 LED light source, and a Hamamatsu Orca Flash 4.0v3 sCMOS camera. mCherry fluorescence was detected using an excitation/emission bandwidth of 577–603 nm/614–659 nm. .. Images were collected using Zen Blue (Zeiss) and analyzed using Fiji ( ).

    Imaging:

    Article Title: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
    Article Snippet: The band intensities for Cis1a and Cis2 were normalized against the band intensity of WhiA and plotted in GraphPad Prism (v9.3.1) with the mean ± s.d. .. For imaging protein localization and fluorescent promoter reporter fusion in S. coelicolor , a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with an sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera and a temperature-controlled incubation chamber was used. .. Images were acquired using a Zeiss Alpha Plan-Apo ×100/1.46 Oil DIC M27 objective with YFP excitation/emission bandwidths of 489–512 nm/520–550 nm.

    Article Title: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
    Article Snippet: The band intensities for Cis1a and Cis2 were normalized against the band intensity of WhiA and plotted in GraphPad Prism (Version 9.3.1) with the standard deviation. .. For imaging protein localization and fluorescent promoter reporter fusion in S. coelicolor , a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera, and a temperature-controlled incubation chamber was used. .. Images were acquired using a Zeiss Alpha Plan-Apo 100x/1.46 Oil DIC M27 objective with a YFP excitation/emission bandwidths of 489–512 nm/520–550 nm.

    Article Title: A Novel Stress Response Pathway Mediates Antibiotic Tolerance and Architecture in Pseudomonas aeruginosa Biofilms
    Article Snippet: .. Time-lapse imaging was started from the beginning of the experiment and images were acquired every 5 min. P. aeruginosa strains were visualized using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7 LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera, and a temperature-controlled incubation chamber. .. Bacterial cells were loaded into B04A microfluidic plates (ONIX, CellASIC), grown by perfusing LB for 6 h in the case of PAO1 WT and Δ batR strains; or perfusing LB for 1 h, then switched to 0.1 mM IPTG LB for 9 h, and LB for 1h in the case of PAO1 strains overexpressing srkA .

    Incubation:

    Article Title: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
    Article Snippet: The band intensities for Cis1a and Cis2 were normalized against the band intensity of WhiA and plotted in GraphPad Prism (v9.3.1) with the mean ± s.d. .. For imaging protein localization and fluorescent promoter reporter fusion in S. coelicolor , a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with an sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera and a temperature-controlled incubation chamber was used. .. Images were acquired using a Zeiss Alpha Plan-Apo ×100/1.46 Oil DIC M27 objective with YFP excitation/emission bandwidths of 489–512 nm/520–550 nm.

    Article Title: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
    Article Snippet: The band intensities for Cis1a and Cis2 were normalized against the band intensity of WhiA and plotted in GraphPad Prism (Version 9.3.1) with the standard deviation. .. For imaging protein localization and fluorescent promoter reporter fusion in S. coelicolor , a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera, and a temperature-controlled incubation chamber was used. .. Images were acquired using a Zeiss Alpha Plan-Apo 100x/1.46 Oil DIC M27 objective with a YFP excitation/emission bandwidths of 489–512 nm/520–550 nm.

    Article Title: DNA damage-induced block of sporulation in Streptomyces venezuelae involves downregulation of ssgB .
    Article Snippet: Received 14 March 2022; Accepted 09 May 2022; Published 15 June 2022 Author affiliations: Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario, M5G 1M1, Canada; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada; Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.. *Correspondence: Susan Schlimpert, Susan.. Schlimpert@ jic. ac. uk; Justin R. Nodwell, justin. nodwell@ utoronto. ca

    Article Title: A Novel Stress Response Pathway Mediates Antibiotic Tolerance and Architecture in Pseudomonas aeruginosa Biofilms
    Article Snippet: .. Time-lapse imaging was started from the beginning of the experiment and images were acquired every 5 min. P. aeruginosa strains were visualized using a Zeiss Axio Observer Z.1 inverted epifluorescence microscope fitted with a sCMOS camera (Hamamatsu Orca FLASH 4), a Zeiss Colibri 7 LED light source, a Hamamatsu Orca Flash 4.0v3 sCMOS camera, and a temperature-controlled incubation chamber. .. Bacterial cells were loaded into B04A microfluidic plates (ONIX, CellASIC), grown by perfusing LB for 6 h in the case of PAO1 WT and Δ batR strains; or perfusing LB for 1 h, then switched to 0.1 mM IPTG LB for 9 h, and LB for 1h in the case of PAO1 strains overexpressing srkA .

    Light Microscopy:

    Article Title: DNA damage-induced block of sporulation in Streptomyces venezuelae involves downregulation of ssgB .
    Article Snippet: Received 14 March 2022; Accepted 09 May 2022; Published 15 June 2022 Author affiliations: Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario, M5G 1M1, Canada; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada; Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.. *Correspondence: Susan Schlimpert, Susan.. Schlimpert@ jic. ac. uk; Justin R. Nodwell, justin. nodwell@ utoronto. ca

    Bacteria:

    Article Title: A bacterial CARD-NLR immune system controls the release of gene transfer agents
    Article Snippet: Protein loading controls were run on 12% SDS-PAGE gels and stained with an InstantBlue Coomassie protein stain (Abcam). .. Bacteria were immobilised on 1% agarose pads and visualised under a Zeiss Axio Observer Z.1 inverted epifluorescence microscope equipped with a Zeiss Plan Apochromat 100x/NA 1.4 Ph3 objective lens, an sCMOS camera (Hamamatsu Orca FLASH 4), and a Zeiss Colibri 7 LED light source. ..



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